Let’s set the scene.

The date is July 27th, 2020 in Boulder City, Nevada. The air, well over 100 degrees, feels like you’re standing in an oven on full blast. The roads are littered with tumbleweeds, like actual tumbleweeds straight out of a cartoon. Surrounded by a desert filled with coyotes, rattlesnakes, and tarantulas. This is what I walked into as a newly recruited intern for the Joshua Tree Genome Project at the USGS.
After losing an internship due to COVID-19, I was desperate, scratch that, very desperate, to find work anywhere I could. Call it fate, divine intervention, or just dumb luck, I received an email late May informing me of a position open in a little town just minutes away from Las Vegas. So, I packed up my Jeep and made the 26-hour drive from Northwest Arkansas to the Mojave Desert, having no idea what I was in for. Little did I know that it would be one of the most rewarding and enriching times of my life.
Over the past 6 months, me and my fellow interns have put blood, sweat, and tears into the Joshua Tree Genome Project. From breaking open pods and counting thousands of seeds in a basement, to working in a greenhouse until 2 am planting those same seeds, our time on this project has been nothing short of an epic adventure. We have spent countless hours mauling over massive excel datasheets, so much so that we began to dream about data, pivot tables, formulas, and Rstudio scripts. We spent days upon days camping in the desert where, much to my extreme delight, I was able to encounter the Mojave Green Rattlesnake (Crotalus scutulatus) for the first time. We camped under the stars and worked in the blazing heat, assessing the growth of plants that interns in previous years had planted. We met incredible people along the way, but none more so than Dr. Lesley DeFalco and Dr. Todd Esque.
Dr. DeFalco and Dr. Esque, or as we now call them, Lesley and Todd, have been the most amazing mentors I could have ever asked for. Through their guidance and leadership, we have been able to achieve things I would never have imagined 6 months ago, and their attention to detail and effective communication skills have left a lasting impression on all the interns. They have been patient, understanding, unfathomably useful, and a joy to work with.
I have nothing but praise for my fellow interns as well. Moving across the country can be a culture shock in and of itself, but add on three strangers from very different locations, the move can seem much more daunting. However, I wasn’t prepared for some of the nicest, smartest, and most hardworking group of people I would have the privilege to call my fellow interns. They have taught me so much and have been a great “quarantine” squad over the past 6 months. My roommate, Nick, has been a constant source of laughter, inspiration, and random nonsense, I a will look back at my time at 629 Avenue L fondly because of him.
My time in the desert has been the time of my life. I have learned from experts, gained some valuable contacts, and made lifelong friends. I look forward to reading the papers that our work will produce, and I can’t wait to see how our findings are used in the future.
Well, that’s all for me, I have lots of work to do, and my time in Boulder City is limited.

Thank you CBG and the USGS for a wonderful experience

Josh

A final salute to the JTGP is in order. This New Year brings about the end of our six month stint with the USGS in Southern Nevada and the Joshua Trees. We have tracked, recorded, organized and outlined the details and info of thousands of baby JTs and are now handing that package of data over to our mentors and the future group of interns, along with a greenhouse full of growing plants with shiny metal tags, ready for out planting into the common gardens.

This internship experience with CBGs CLM program was round two for me (did an internship with the FS in Idaho in 2019) and while my first internship was a fantastic opportunity in plant conservation, management and all things botany, this JTGP internship has been a superlative introduction and lesson in botanical research. It has been an incomparable hands-on experience involving research design, time-management and troubleshooting, data collection efficiency and a dip into the statistical world.

As an individual who is intending on graduate school (and the inevitable design and execution of my own work), this internship was a much needed segue into the world of big time botanical research. I am now aware of best practices for managing study plants and just how much time and thought must go into all research. I have also experienced the struggles, set-backs and wrong turns, and have learned how to approach them with flexibility and curiosity. And most of all, I have learned the true value of working in a team. My JTGP co-interns have been with me through every one of our planning moments, hiccups, and victories. I have realized how incredibly refreshing it is to discuss courses of action with individuals who think differently and I know that we have been able to accomplish all of the work we did these past six months because of the constant support and healthy challenging that occurred as we worked together with these plants.

I also have to mention that being mentored and guided by top-tier research scientists at the USGS has been so so SO awesome. Receiving endless guidance and confirmation from Lesley and Todd has helped me to better analyze and understand the flow of this research project. I have tucked away many gold nuggets of advice and suggestions for use in my personal and professional life. They have challenged us as a group, and me as an individual, to truly dig in and open myself up to the new experiences and lessons that the JTGP had to offer.

So, it is with pride and excitement that I close this desert flavored chapter of my life. I’ll be checking in over the years to see how the JTs are doing and am so grateful to the CBG and USGS that I was able to play a roll in this amazing research 🙂

One week ago, I drove the rickety bridge over Brown’s Run for the last time. I turned right, past the field of ironweed and wingstem aster, past the horse pasture and the barn, then turned left toward Huntersville, heading east on Route 39. My 4Runner was packed to the gills, weighted down with several new additions since I arrived in Marlinton in July. Atop the center console was my newest friend, Moona, a young stray cat who adopted me on a camping trip at Tea Creek in late November. In my cooler was a bag of frozen foraged chantarelles, remnants of the final mushroom haul in October. Stuck between the pages of field guides and biology textbooks were pressed herbarium specimens of Dryopteris intermedia and Athrium angustum, which I picked up on a botany survey in September. Packed away in a small box at the bottom of the heap were botanical drawings of some woodland flora, which I made while memorizing species in August. I was driving away from Marlinton, loaded down with five months’ worth of gifts from my experience there.

Two of the most impactful gifts from this experience in the Monongahela could not be packed away in my truck. For one, I found a lifelong friend in my co-intern, Ivy. A top-class work partner, a talented biologist, and a supportive sister, Ivy brought immeasurable joy and depth to my time in Marlinton. We both arrived in Marlinton in July as strangers, in the middle of a pandemic, with little direction around how to begin our project. Together, we pushed one another to memorize local species and hone our botanical vocabulary, design and build a seed processing station in the bunkhouse garage, and process over 100,000 seeds. We drove for hours together, commiserating with one another about the state of politics, co-created botany career goals, and encouraged one another in personal growth. We belted the lyrics to classic tune, danced along roadsides while collecting berries, threw piles of rainbow autumn leaves into the air, and braced against winter winds together atop the Dolly Sods ridgeline. Ivy’s presence brought such delight, inspiration, and comfort to this internship experience for me. We plan to continue to support one another for many years to come as we dive into the next step of our botany careers.

The other intangible gift from the Monongahela was a clear vision of a career in field botany. Working at the Marlinton Ranger Station and at Appalachian Headwaters’ native plant nursery provided me glimpses into career possibilities that fit my skillset and interests, but none of those pathways captured my passion as did botany surveys. The first day I went out on a botany survey with the South Zone field botanist, Emily Magleby, I knew I had found my dream job. Before this internship, I had a glimmer of an idea of what a career in conservation and plant work would look like. Now, having shadowed Emily and the other botany technicians (Ken Hiser and Todd Kuntz) on botany surveys and seed collection days, I have had the opportunity to learn about multiple career paths that lead to the same end of working outdoors, with plants. Thanks to mentorship and story-sharing from these botanists, I now have a clear idea on how to refine my skills and knowledge in order to pursue my dream of outdoor, botany-focused conservation work. I can now streamline my career goals and build on precisely the skills I need to work as a field botanist.

With the new year swiftly approaching, I carry with me the gifts of the Monongahela, both tangible and immaterial. This internship experience has left me with a new cat, a solid career network, several mentors, a specialized set of botany skills, and a sense of clarity about where I will be headed. I am unusually enthusiastic about filling out job applications, feeling supported by my new people and finally qualified for the wide array of job possibilities that await me.

A huge thank you to my mentors (Amy, Cindy, Emily, Ken and Todd) and to Ivy for the learning experiences and encouragement during this difficult season. I couldn’t have done it without you.

It is hard to believe that we are already halfway through December, which means we have one week left in Marlinton before we head home and work remotely until the new year. We have collected more than enough seeds from the available populations from our species list and are spending our final weeks here processing the last few buckets-full of American mountain ash, ironweed, Clematis virginiana, closed-bottle gentian and smooth alder. We may still go out collecting, but only for those seeds which persist into the winter and require minimal processing time: ironweed, staghorn sumac, C. virginiana. By the end of next week, we will have delivered all of our perennial woody plant seeds to Appalachian Headwaters where they will be stored and propagated over the next year. Our annuals will be stored here in Marlinton, since they can be cultivated in situ through wind dispersal and scattering.

We have collected thousands upon thousands of seeds during these past few months. It is quite amazing to see the totals: 14,000 seeds of alternate-leaf dogwood, over 15,000 seeds of closed-bottle gentian, and 15,000 of southern mountain cranberry. Even for species that were scantly populated (catberry, for instance) we’ve collected over a thousand seeds. Our repository will serve as a reservoir of plant life used to pioneer forest succession on strip mines in the Monongahela for years to come. In turn, the plants that grow from our seeds will impact this forest for centuries. Heck, millennia! Theoretically, the seeds we’ve collected will act as the first catalysts in the succession of an enduring red spruce forest. These plants can withstand young soils and grow in direct sunlight, and over the next decade they will morph the rocky restoration site into a haven of biodiversity. Birds and other pollinators will consume and disperse the second generation of seeds across the site, reallocating nutrients to the stripped, rocky soil and forming essential foundations for early-succession tree species. As large trees take hold in the newly rich soil, they will shade out the earliest pioneer species from our seed stock, forming an early-succession broadleaf forest. Eventually, red spruce will grow up in the shaded understory of this forest, transforming its soil composition into a deep humus layer filled with mycorrhizal fungi that push out early pioneer species and favor the dominance of late-succession coniferous tree species, and plants that can survive in the dark understory.

One must zoom out at least one hundred years to see how our daily efforts will endure. Three generations from now, visitors may come across Sharp’s Knob and walk upon a cushion of moss and knee-deep duff teeming with a root network of ectomycorrhizal fungi. Maybe they will breathe the cool, terpene-scented air found only in a copse of red spruce, their eyes comfortably shaded by the dense canopy above. Perhaps they, too, will collect fruits, but instead in the form of Vaccinium berries, mayapples, mushrooms, spruce cones. And on the way out, they’re bound to drop a few on the ground, dispersing the next generation of forest flora.

And the cycle continues…

Good news from the JTGP CLM team! We are gearing up our baby Joshua Tree’s for their final transplant into the Common Garden sites across the Mojave. These little ones have come a long way and look very official with their field-ready metal tags.

We still have some maternal lines that are struggling to grow and it will be interesting to see if they pull through or if they simply won’t get to Gardens. As we all know by now: plant research is a dynamic and patient process! All tagged JTs will be transplanted in the gardens over this winter and will have at least a year to grow and develop in their given environments before JTGP leading scientists start to collect genetic and phenological from them (While my group of CLM JTGP interns will be long gone by then, I am excited to check in with our PIs and hear how everything is going in the Gardens).

Because our time here is quickly coming to an end our team has also been super busy organizing, cleaning up and structuring the many data we have been collecting over the past five months. We’ve mastered excel and dabbled in R and are continuing to develop and analyze the loads of info we have on the germination and growth of our JTs.

I can’t believe my time with the BLM is coming to an end! It seems like I only just got to Boise, and now I am embarking on a road trip back home to Austin. I am currently writing this post from Ohlone land, in the ancestral territory of the Muwekma and Ramaytush Ohlone (otherwise known as San Francisco). I have also been wrapping up some data management and writing for the office remotely the past couple of weeks.

Even though it seems like time has flown by this summer, I leave Idaho with so much new knowledge and excitement. While I started this internship with no prior experience in the sagebrush steppe and with minimal exposure to land management, I leave acquainted with the plants of this ecosystem and feeling much more prepared to continue this work in the future. Towards the end of my internship, I even met a former CLM intern who was just hired for a full-time position in my office. It was so sweet to chat about our experiences in the program and spend time in the field together.

Even with COVID posing unique challenges, I still experienced a wide variety of life in a BLM field office: from conducting veg surveys and searching for rare plants to fence construction and riparian assessment to data analysis and writing reports. I was also able to meet permittees and see what it is like to do community outreach in the field office. I even tagged along with our geologist to experience the mineral program. I am so glad for the opportunity to intern with such a welcoming group– my mentor and all of my coworkers were so incredibly kind and knowledgeable and made me feel truly like a part of the team. It was great to work alongside them and learn about all the different parts of the field office.

The past five and a half months have been a period of growth and challenges. In such a turbulent time filled with national and international grief and uncertainty, I am incredibly grateful to have been employed doing work that I love and find meaningful. I will miss the views of the mountains and working with the plants of the high desert. Idaho is a beautiful place, and the Owyhee Field Office had so many sites to explore and plants to meet! I hope that I will be able to come back and visit in the future. Next year’s Botany conference will be in Boise (hopefully, although who knows with COVID), so I might be back in the summer.

I am not yet sure what is in store for me over the next few months, but I fully intend to spend it with loved ones and botanize as much as I can.

Until then,

Lili

Happy late October from Southern Nevada. The JTGP team is basking in balmy fall weather here and working away in the USGS greenhouse, pampering our Joshua trees. You read right: pampering. Who would have thought these magnificent plants of the Mojave would be so fussy!

You see, it turns out that some of the seeds are quite particular and have decided to fight germination with stark determination. Tucking them into nice damp soil, regulating their day and night temperature in the greenhouse, and diligently watering them makes not a difference.

They simply weren’t having it.

But, little did they know, the same thing could be said for us: we were not having their complete lack of germination.

So, we decided to treat these most persnickety seeds like the royalty that they are: laying them out on a damp filter paper in a Petri dish and setting them in a temperature regulated growth chamber to imbibe in warm darkness. We visited them twice daily to alter the chambers day-time and night-time temperatures, gently cleaned them if mold popped up, kept each filter paper nice and damp, and crossed our fingers.

And what do you know-it was just what they preferred! In no time we had lots of happy germinates.

Now, every day more and more germinates appear in the Petri dishes and we scramble to transplant them into their respective plant bands (tall, bottomless cardboard ‘pots’ that allow the Joshua tree’s roots to grow long). And our success hasn’t stopped there. These Joshua trees are (finally) pleased enough to send up bright green hypocotyls and cotyledons. Up next are primary leaves and more, each plant growing as much as possible before its final transplant into the Common Garden sites at the end of the year!

And now we know: despite initial struggles, germination is indeed possible. So grow on Joshua trees, grow on.