The Hunt for ERUM

There’s much to talk about here at the Rocky Mountain Research Station in Boise, ID! In the short two and a half months since I’ve began this position my partner and I have been darting all over the Great Basin involved in some cool research. Most of our time these days is dedicated to searching for a particular plant species, but we have also gotten involved in some smaller projects that needed extra hands.

Though first, I suppose I’d like to talk more about the Great Basin itself. Despite growing up in Colorado, and seeing parts of the greater Great Basin ecosystem, the basin isn’t something I have thought of very much. In fact, it took several hours of unbroken driving throughout this region to really appreciate its magnificent vastness, like a rolling sea of scrubland mottled with pinyon-juniper woodland in-between stark mountain ranges. Much like the sea, the magnitude of the apparently desolate land is intimidating, yet amazing.

A view from the top of Yellow Peak in the Sheldon National Antelope Refuge in Nevada.

The Great Basin is defined primarily by the fact that the rivers flowing through this region do not drain into any major ocean or worldwide system. The water that enters the Great Basin, stays in the Great Basin (and now I know where Vegas ripped their slogan from!). This region is dominated by scrubland and pinion-juniper, but is home to a wonderful suite of forbs, one of which has been our primary focus for the past month, ERUM. ERUM stands for Eriogonum umbellatum, or sulphur buckwheat, a perennial from the family Polygonaceae. My field partner and I spend most of our time traveling to locations with presence records of this species, and collecting leaf tissue, herbarium vouchers, and seed from them when available.

Eriogonum umbellatum.

These materials are raw inputs into a research pipeline. Genetic material and phenological information are used to characterize varieties of this species while seeds from various climates are grown in several so-called “common gardens” across the Great Basin. All of this information gets united in an effort to identify “seed zones” for ERUM and its many varieties. These zones are areas throughout the Great Basin associated with particular environments and climatic conditions which result specialized in adaptations in ERUM. For instance, a sample of ERUM seed collected in a high-altitude forest meadow zone would likely not grow well in a low-elevation scrubland zone, and vice versa. So by identifying these seed zones, and characterizing the seed collections by said zones, restoration projects can use this information to select ERUM seed suited for the proper climate and environment. Developing large quantities of native seed is an extremely expensive process, and much seed can go to waste if the environment isn’t suitable. My mentor, Jessica, mentioned to us that after all the labor, permits, equipment, and resources, a bag of seed can be worth more than its weight in gold!

Hunting for ERUM feels like one great scavenger hunt, and it’s always a bit of a rush to stumble upon some. My time so far in this position has been pleasant. I feel very fortunate to travel to so many breathtaking places I likely never would have gone to otherwise. The great outdoors sure has a way of making one feel whole…

As we transition from traveling around the Great Basin collecting seed to setting up common gardens, I hope learn whether development of a reference genome is in the works, what sort of genes are being used as markers to identify varieties, as well as some general curiosities about the potential link between plant breeding, agronomy, and restoration.

Stanley Lake, ID
Jackson, WY

Greenhouse Planting Party

October snow in the Spring Mountains!

Seedlings, snow, and Santa Rita mountains! It’s been a busy month out here in the desert, planting thousands of delicate Joshua Tree seeds; I’ve been organizing, prepping petri plates, germinating, and finally gently nestling these fellas in the soil mixes I described last blog post. All in all, this process is fairly arduous and complex, but the seeds themselves make it a lot easier. As soon as they are set in a little pool of water, they suck it up rapidly and most start producing a root within 2-3 days! A couple weeks into this task, we already have many of our early planted trees starting to emerge with their bright green leaves above the surface. The goal of this planting is to have about 4 plants per genetic line in each of our 4 sites. Sadly, I will be wrapping up this internship before USGS plans to plant these seedings in the desert, but in the meantime I will continue to plant seeds, care for the seedings, and make sure everything stays organized!

Joshua Tree seeding ~2 weeks after planting

Besides using my green thumb in the greenhouse and the usual Joshua Tree field work, I’ve also been asked to start helping with a couple projects. This week, I will be going into the field to help take cuttings of a desert shrub called Eriogonum fasciculatum. We will be taking branches and leaves from wild bushes in a bid to grow cuttings from them. Next post I will go into more depth with this project and the field work, along with whatever else interesting comes up!

Golden Eagle I saw out in the field in CA

Lastly, I took a trip down to the Santa Rita mountains in SE Arizona! These mountains are known as “sky islands” where interesting birds that wouldn’t be found elsewhere in the desert can be spotted. Below are some photos of things I saw in the area and on the way there and back! Until next time, thanks for reading!

Saguaro Cacti mean welcome to the Sonoran Desert. Adios Mojave!

First Month in the Mojave!

Adult Joshua Tree in Red Rock Canyon

It’s been one month since I moved to Las Vegas to work with the US Geological Survey, and I’ve already seen and done so much! I have just about completed two rounds of field work. We travel across the Mojave every three weeks, visiting four “common gardens” in three states. The project I am mostly focused on is called the Joshua Tree Genome Project, and on these trips we inspect hundreds of Joshua Tree seedlings. These gardens vary in terms of climate and soil, but no matter what, it’s still very hot! I learned very quickly that working in extreme heat, even in the early morning, requires serious preparation and management. I wear long sleeves and a big sun hat, and hydrate very frequently. We also have to watch our salt intake to make sure we aren’t displacing electrolytes too quickly. That said, it is super cool to work in an environment so starkly different than my humid and forested home state of Virginia. One other aspect of field work that is a very new experience to me would have to be watering. We tow a giant water tank trailer to our gardens, and use an engine and fire hose to shower the seedlings with a lot of water. Its certainly a little more involved than using a garden hose, especially when the wind blows the water right back at you and soaks you completely! Next week I finish this round of fieldwork, which will entail sleeping overnight in the field, so that we can water and work in the cooler evening and morning.

Watering the seedlings

Outside of fieldwork, I have spent quite a lot of time with Joshua Tree seeds. This past month, I have counted thousands of seeds, both to give us an idea of our inventory, and to partition off seeds for future projects. Now that I have finished with the genome project seeds, I have been fiddling around with Python, both with the seed count data and the field data. My third realm of work would be greenhouse clean-up. Not necessarily as exciting as field work, but it is a nice break from data entry, and we get to repot Joshua Tree seedings, which is pretty cool! Overall, I’m glad I’ve been able to work a variety of duties, gaining experience in both office-work and field-work. Eventually, I will also be working in the lab, which I definitely look forward to.

Joshua Tree (Yucca brevifolia) seeds being sorted

One last thing to mention is the nature around here in Nevada! Coming from Virginia, the only lizards I was accustomed to seeing were skinks, but out here, there is a myriad of cool reptiles! I’m also lucky enough to live near a wetland preserve, where I have seen a variety of new birds! As well, I have seen so many cool desert and mountain flora, including cacti and adult Joshua Trees! Recently, I have learned that there are several endemic species living in the Spring Mountains, just outside Vegas, including the very cute Palmer’s Chipmunks!

Western Fence Lizard (Sceloporus occidentalis)

Moving forward, I am excited to continue with field work, begin lab work, and start to really analyze our data! Of course, I also look forward to exploring more of the Mojave and its amazing wildlife!

Palmer’s Chipmunk (Neotamias palmeri)

The Joys of Research Under the Desert Sun

Like a well oiled machine headed down Route 66, we worked through another meeting regarding the Joshua Tree Genome project (JTGP). After hashing out questions, and weaving through theoretical discussions with an almost religious fervor, we finally felt ready to disconnect from our mentors and carry out our plan, and so we did. And in that single click, a wave of relief brushed over us momentarily as the tension of another video call rescinded. But this post-collaboration satisfaction came crashing down as soon as we opened our mouths again to reveal that all four of us interns had a completely separate and completely different understanding of what we had just agreed to do over the call.

“I think I’m just gonna call Lesley back”

“Thanks Olivia”

CLM Interns Michele Beadle (left) and Olivia Turner (Right) scrupulously working through our collected germination data in search of discrepancies

The above passage highlights some of the joys engendered by botanical collaboration under the desert sun. And despite its sweaty faults and confusing twists and turns, we still consider it fun. Fun in an esoteric “type-2” sense of the word, but fun none-the-less! This week’s “fun” has coalesced itself in the form of 3 main lessons.

Lesson #1: Sorghum cotyledons look pretty similar to Joshua Tree cotyledons. We had been enthralled with the idea that one of our mighty seedlings was growing so quickly! The seedling in question was one of the first plants we were able to observe as the germination experiment was just getting off the ground. In excitement we called over one of our mentors, Todd Esque, to show him our photosynthetic anomaly! Looking first to the plant, and then back to me, he couldn’t help but let out a gentle chuckle before asking why we were growing crops in the greenhouse. In hindsight it should’ve been obvious we were growing a member of the grass family, but hindsight always runs 20/20 (as we all know).

Two very healthy Joshua Tree seedling cotyledons
Our healthy sorghum plants serve as evidence that if research doesn’t work out, perhaps agriculture would be a successful venture for the 4 of us

Lesson #2: Joshua Trees are plants of the desert, and probably should not be sitting in pools of water. During our stint as sole operators of the greenhouse whilst our mentors took a much needed break, we followed a strict schedule. Water the crates every morning at 8:00 AM, and water more lightly every evening at 4:00 PM. We ritualistically followed these guidelines given to us, without even considering the warning signs of over watering. Some of these warning signs included the rust orange pools of filth gathering underneath our crates, the intensifying humidity of our work environment, and of course the emerging fungal “snow cover” noted upon the substrate of some of our plants. Luckily for us our head mentor, Lesley Defalco, pulled the plug on our 5000 attempted murders, and as a result our plants did not spend enough time swamped to be significantly damaged! We now follow a more conservative practice of watering only every other day, which saves water and cuts our work time as well!

A look at our crates, which will soon house 3200 young Joshua Trees

Lesson #3: We truly are in good hands here in Boulder City, Nevada. The work down here has been extremely rewarding, but it hasn’t been without its difficulties. Keeping up with the germination of 5000 plants, and then working to transplant well over 1000 plants all while tracking every seed meticulously on a daily basis has proven difficult for a team of 4. Add on top of this consistent temperatures of 110+ Fahrenheit, and no days off for 2 weeks straight, and you might get a disgruntled group of botanical goblins! But, while I would still entertain the label of botanical goblin, I would not say we became disgruntled, and this is because our mentors, sympathetic to our struggles, ordered that we take two days off in the middle of the week and recharge. Research is difficult, and working to create the best data possible for such an incredible and important project is a stressful proposition. But we 4 interns feel confident that, when push comes to shove, our mentors, the JTGP team, and The Chicago Botanic Garden truly have our best interests in mind.

All smiles from “The City that Built The Hoover Dam” as Michele Beadle transplants a healthy Joshua Tree seedling to its respective crate

That’s all for this week from the desert!

Stay Cool,

Nicholas Filannino

Botanical Exploration with Jerry Theim

The first real botanical adventure I experienced was in the Calico Mountains Wilderness on an expidition lead by Jerry Theim. Few people would think to place botany and adventure next to each other. But in Nevada’s Black Rock Desert, where the terrain is a product of a unique geologic history and as rugged and rocky as anything, searching for rare species is anything but boring.

We met Jerry for the first time at a fork in the road, and as we climbed into his truck we made our acquaintances quickly. Being a very talkative man, he dove into explaining what we would be doing with him that week. His plan was to spend the next four days hiking through out the wilderness, checking various points of interest he had scoped the day before.

As we drove he pointed out the vast geologic expanses, telling us names of the rare species that live there. Steam boat mountain, where Caulanthus barnedbyii was recently found, and the Jackson range, where he spent a week during the last field season searching for a rare Pentstemon species. It was clear that he knew the area well. We pulled onto a 2 mile an hour road and drove up to the base of a canyon that was carved out by a long dried up water flow. “Let’s go botanize!” He exclaimed and we got together our gear for a day of hiking.

Being from Massachusetts, I had never seen anything like the range we were about to venture into. The Calico Mountain wilderness is a vast expanse of multi-colored slopes, most of them very steep and covered in tallus. We set out trailblazing through the canyon, and we approached a dark and ominous slope covered in tallus. I had never even heard of tallus before, let alone climbed on it. I was quite surprised as we began our ascent up the 60% slope of sharp, loose, MINERAL? that slipped and slid as we scrambled our way to the top.

We were headed for an ash deposit a the top of one of the smaller peaks of the bizarrely pigmented mountains. This one was splotched with green from oxidized copper, and brown and pink from other minerals. The ash deposit at the summit was bright white and is a unique soil type endemic to the Black Rock Desert-High Rock Canyon Emigrant Trails National Conservation Area.

Jerry was a pro climber. I learned that day, among many other things about Jerry, that he is a native of Nevada and an avid outdoorsmen. Unsurprisingly he found his way to the top with ease. He is a modest man, and it was not until our last day out that Jerry told us of his botanical accomplishments. Apparently he was something of a local celebrity in the Great Basin plant community. In his exploration of Nevada he had found an abundance of species new to science, many of which now share his name.

During our climbs he told us stories of Arthur Cronquist and his time collecting for herbarium collections in NYC and Cambridge. He was also responsible for many of the collections that made possible the publication of Intermountain Flora, one of the best dichotomous keys for the Great Basin area. He even shared with us what he did when he was not botanizing- limosene driving for the casinos in Reno and carpentry.

He trained us in the methods he used for botanizing rare species, and gave us heaps of plant names common to the area. It was hard to take it all in, and our field books had many new pages filled with species lists. He also shared with us tips for navigating the area, and gave us valuable advice on survival in the desert.

On the way back from the trip, I was struck with an overwhelming sense of gratitude for having been given the opportunity to botanize with Jerry. His training served us well in later inventories for rare species of the area. I will always be thankful for participating in the CLM internship for this reason, and many others.